Assessment of genetic diversity in different geographically isolated Alhagi graecorum Boiss. populations using SCoT marker

Document Type : Original Article

Authors

1 Botany Department, Faculty of Science, Ain Shams University, Cairo, Egypt.

2 Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center,, Giza, Egypt.

3 botany department, faculty of science,ain shams university

4 botany department, faculty of science, Ain shams university.

Abstract

Abstract: The Alhagi genus (commonly known as Camel thorn) belongs to family Fabaceae, has various medicinal properties as revealed by several studies in some diseases treatment. The assessment of genetic diversity within and among Alhagi graecorum populations was carried out using ten SCoT primers. In this study, 25 individuals (five individuals from each population) were sampled, amplified separately and as bulked DNA (DNA from the five samples gathered from each population were bulked together to identify polymorphism among the studied populations). In total, 140 and 156 bands were scored with 71.3% and 63.7% polymorphism, respectively. Average polymorphism information content (PIC) for amplified DNA individual of samples was 0.87 and bulked- DNA samples was 0.75. Analysis of molecular variance (AMOVA) showed that 52% of the total variation was observed within populations while variation among populations was 48%. The UPGMA cluster analysis divided the 25 individuals and bulked samples into two main groups, each group was further subdivided into many sub-clusters. PCoA was also carried out and in agreement with the UPGMA. The GC values of sequencing for three clearly unique bulked- DNA bands from (SCoT5, SCoT20 and SCoT21) were 54.7, 45.5 and 60.6%, respectively with average percentage 53.6%. The present results showed the efficiency of SCoT markers for the evaluation of genetic relationships between and among populations due to their efficiency in revealing polymorphism.

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