The relationship among 15 samples representing 9 species and 8 sections of Plantago, based on electrophoreticseed protein profile , as well as karyotype data is discussed in the light of their current sectional and sub sectionaldelimitation. The delimitation of P. lanceolata as a separate identity from P. afra is congruent with thetaxonomic differences between these two species,while its separation from P. amplexicaulis and P. cylindricamatches the cytological differences between them. The separation of P. alpina and P. coronopus based on theelectrophoretic pattern of their seed protein is congruent with the chromosomal differences between them, P.alpina is a tetraploid with 2n=24 and a base number of X=6, whereas P. coronopus is diploid with 2n=10 andX=5. The delimitation of P. afra supports its taxonomic separation from the other studied species. The pattern ofprotein electrophoresis under reducing conditions distinguished P. major from the remaining species. Thisseparation is supported by the delimitation of this species in a separate section i.e. Plantago. The separation of P.media from P. amplexicaulis and P. cylindrica by the analysis of protein pattern, under reducing condition,confirms their separation based on morphological and chromosomal differences between them. Under nonreducingcondition P. amplexicaulis and P. cylindrica are separated together as one group distinct from P. majorand P. media which are delimited as another group