@article { author = {mahdy, mayada and hamdy, rim and Mohsen, Loutfy and Badr, Abdelfattah}, title = {Description of seed and pollen micromorphology and their taxonomic impact in some Solanum L. species}, journal = {Taeckholmia}, volume = {39}, number = {1}, pages = {1-17}, year = {2019}, publisher = {Cairo University Herbarium (CAI)}, issn = {1110-7413}, eissn = {2357-044X}, doi = {10.21608/taec.2019.11353.1003}, abstract = {Seed micromorphology, of 17 species and pollen micromorphology of 16 species of Solanum L. (Solanaceae) were examined using both light and scanning electron microscopy (SEM) to determine the significance of seed coat features and pollen characteristics as taxonomic characters. The species, used in this study, represent three subgenera and 11 sections of Solanum in Egypt. Macro and micro-morphological characters including seed shape, size, colour, surface; hilum shape, size, position, level; seed coat appearance, cell wall; shape of pollen in polar and equatrorial view, colpus membrane and apices and exine ornamentation have been described and their taxonomic impacts have been outlined. Seed shape was either obovate or reniform and seed size ranged between 1.1and 3.8 mm in length and 0.9 to 3.1 mm in diameter. Four types of seed coat appearance have been described: reticulate, cerebelloid, striate and without conspicuous cell lumen and six types of pollen shape were found in the studied species: prolate, subprolate, spherical, prolate-spheroidal, oblate-spheroidal and perprolate. Pollen length in polar view ranged from 15 µ to 34 µ but in the equatorial view it ranged from 9.1 µ to 25 µ. Four types of exine ornamentation have been recognized: scabrate, psilate, granulate and verrucate. Nineteen characters of seed and pollen micromorphology features proved useful in the construction of a dichotomus indented systematic key for the examined species. The major remarks are the differentiation of S. melongena, S. schimperianum, S. forsskaolii and diphyllum by their reinform seed shape from the remaining species which have ovoid seed shape. Hairy seed surface distinguishes Solanum lycopersicum, S. seaforthianum, S. nigrum and S. vilosum. Seed coat colour, appearance and pollen shape were useful traits for the identification of other nine species.}, keywords = {Pollen,seeds,SEM,Solanum species,Taxonomy}, url = {https://taec.journals.ekb.eg/article_47327.html}, eprint = {https://taec.journals.ekb.eg/article_47327_7a8462c69400263d388f13c2179e370e.pdf} } @article { author = {elmidany, maha and Galal, Tarek and Farahat, Emad and Mohsen, Loutfy}, title = {Phytosociology of rainfed barely along the western Mediterranean Coast, Egypt}, journal = {Taeckholmia}, volume = {39}, number = {1}, pages = {18-33}, year = {2019}, publisher = {Cairo University Herbarium (CAI)}, issn = {1110-7413}, eissn = {2357-044X}, doi = {10.21608/taec.2019.16639.1005}, abstract = {The exponential decrease of precipitation and increase of temperature in many dry regions in the world affect negatively the growth of main crops. The presence of associated weed species with these crops may add more adverse impact on yield. The present study aims at investigating the phytosociological structure of its associated wild species with rainfed barely (Hordeum vulgare L.) at Marsa-Matrouh Governorate, Egypt. Forty-eight stands were assigned at Marsa-Matrouh city for estimating the number of associated species with rainfed barely and its visual cover. Seventy-five species belonging to 63 genera and 24 families were recorded as associated species with rainfed barley. The most dominant families were Asteraceae followed by Poaceae, Chenopodiaceae, Fabaceae, Brassicaceae. A total of 48 species were recorded as annuals. On the other hand, 25 species were perennials. Two species were biennials. Therophytes were the dominant life form. The application of TWINSPAN on the cover estimates of 75 associated species recorded in the 48 sampled stands of barley, led to recognition of eight vegetation groups.The main indicator species for vegetative groups (1, 2, 3, 4, 5, 6, 7 and 8): Glebionis coronaria, Carrichtera annua, Elymus farctus, Erucaria microcarpa and Malva parviflora are native species at the Mediterranean coastal strip. Carrichtera annua-Erucaria microcarpagroup (VG 3) had the highest value of species richness. Moreover, Erucaria microcarpa- Vicia monantha (VG 6) showed the highest value of species turnover. Erucaria microcarpa- Vicia monantha (VG 6) showed the highest value of shannon index. Elymus farctus- Erucaria microcarpa (VG 5) showed the highest value of Simpson index. The present study will help in improving the understanding for the phytosociology of rainfed barely and will help in improving the agricultural practices to increase the yield of this plant under the prevailing harsh conditions.}, keywords = {Phytosociology,Rainfed barely,species richness,Marsa-Matrouh,weeds}, url = {https://taec.journals.ekb.eg/article_52426.html}, eprint = {https://taec.journals.ekb.eg/article_52426_0752100895dd196f61f5a9d2a56aa63c.pdf} } @article { author = {Shamso, Eman and Sadek, Ahmed and Hosni, Hasnaa}, title = {Morphological and anatomical characteristics of endemic Rosa arabica ( Rosoideae, Rosaceae) from Sinai, Egypt.}, journal = {Taeckholmia}, volume = {39}, number = {1}, pages = {34-43}, year = {2019}, publisher = {Cairo University Herbarium (CAI)}, issn = {1110-7413}, eissn = {2357-044X}, doi = {10.21608/taec.2019.17752.1006}, abstract = {Rosa arabica is endemic to the high mountain area of the St. Catherine Protectorate, Southern Sinai, Egypt. It has been listed as Critically Endangered in the IUCN Red Plant List. The present study aimed to provide detailed morphological and anatomical characters of the endemic Rosa arabica by using light and scanning electron microscopy in order to expand knowledge of its taxonomy. The present study revealed the presence of three types of trichomes: simple unicellular, glandular unicellular stalked and glandular multicellular multiseriate. Anomocytic stomata, occasionally hemiparacytic and actinocytic were observed mostly on abaxial surface. Epidermal cells were smooth, with dispersed wax granules and stomata slightly raised with outer stomatal rim. Druses were also reported in pith and cortex of stem and petiole. Achene micromorphology showed scalariform pattern with anastomosed raised anticlinal walls and depressed periclinal walls. These data are presented here for the first time and their taxonomic values are discussed.}, keywords = {Achene,anatomy,Egypt,Endemic,Rosa arabica}, url = {https://taec.journals.ekb.eg/article_55848.html}, eprint = {https://taec.journals.ekb.eg/article_55848_5e71343ecf6b2342886db6ebbba329ab.pdf} } @article { author = {Abd El- hak, ghada and darwish, shafik and halim, Atef and zaki, Hosam eldin and rayan, walaa}, title = {Assessment of genetic diversity in different geographically isolated Alhagi graecorum Boiss. populations using SCoT marker}, journal = {Taeckholmia}, volume = {39}, number = {1}, pages = {44-66}, year = {2019}, publisher = {Cairo University Herbarium (CAI)}, issn = {1110-7413}, eissn = {2357-044X}, doi = {10.21608/taec.2019.19481.1008}, abstract = {Abstract: The Alhagi genus (commonly known as Camel thorn) belongs to family Fabaceae, has various medicinal properties as revealed by several studies in some diseases treatment. The assessment of genetic diversity within and among Alhagi graecorum populations was carried out using ten SCoT primers. In this study, 25 individuals (five individuals from each population) were sampled, amplified separately and as bulked DNA (DNA from the five samples gathered from each population were bulked together to identify polymorphism among the studied populations). In total, 140 and 156 bands were scored with 71.3% and 63.7% polymorphism, respectively. Average polymorphism information content (PIC) for amplified DNA individual of samples was 0.87 and bulked- DNA samples was 0.75. Analysis of molecular variance (AMOVA) showed that 52% of the total variation was observed within populations while variation among populations was 48%. The UPGMA cluster analysis divided the 25 individuals and bulked samples into two main groups, each group was further subdivided into many sub-clusters. PCoA was also carried out and in agreement with the UPGMA. The GC values of sequencing for three clearly unique bulked- DNA bands from (SCoT5, SCoT20 and SCoT21) were 54.7, 45.5 and 60.6%, respectively with average percentage 53.6%. The present results showed the efficiency of SCoT markers for the evaluation of genetic relationships between and among populations due to their efficiency in revealing polymorphism.}, keywords = {Key words: Medicinal plant,Alhagi graecorum,Genetic variations,Sequencing,SCoT}, url = {https://taec.journals.ekb.eg/article_63874.html}, eprint = {https://taec.journals.ekb.eg/article_63874_7451a9e82351b0fc5ff09cfecf3889ca.pdf} } @article { author = {Abd El- hak, ghada and darwish, shafik and halim, Atef and Hassan, Hussam and rayan, walaa}, title = {Genetic variation within and among the wild populations of Alhagi graecorum using ISSR markers}, journal = {Taeckholmia}, volume = {39}, number = {1}, pages = {67-85}, year = {2019}, publisher = {Cairo University Herbarium (CAI)}, issn = {1110-7413}, eissn = {2357-044X}, doi = {10.21608/taec.2019.19054.1007}, abstract = {Abstract: Alhagi graecorum Boiss. (Camel thorn) (Family Fabaceae) is a wild plant which grows in salty soils and dry environment. It is native to Mediterranean and central Asia. Several studies have revealed the use of Alhagi plants in treating a wide spectrum of diseases. In this study, eight ISSR primers were used to assess genetic diversity of five populations of A. graecorum (El-Dakhla Oasis; Botanical garden of botany department, Ain Shams University; Wadi El-Rayan; Qarun Lake and Siwa Oasis) using individual- and bulked samples based approaches. Twenty five individuals were sampled, amplified separately and amplified as bulked DNA to identify polymorphism among the studied populations. In total, 101 and 97 bands were scored with 83.3% and 78.4% polymorphism, respectively. Average polymorphism information content (PIC) for amplified DNA of individual samples was 0.875 and bulked-samples was recorded 0.69. Analysis of molecular variance (AMOVA) showed that 63% of the total variation was observed among populations while variation within populations accounted for the remaining 37%. The highest genetic similarity index was (0.99) among the studied Alhagi genotypes was recorded between the two genotypes 6 and 7 both individuals were belonging to Ain Shams University population, while among inter populations was (0.853%) between Qarun Lake and Siwa Oasis populations. The UPGMA cluster analysis divided the 25 individuals and bulked samples into two main groups. Each group was further subdivided into many sub-clusters, PCoA was also carried out and in agreement with the UPGMA. The obtained results demonstrate that the ISSR markers were efficient for evaluation of the genetic relationships between intra- and inter-population due to their efficiency in revealing polymorphism.}, keywords = {Key words: Medicinal plant,Alhagi graecorum,Genetic diversity,molecular markers,ISSR}, url = {https://taec.journals.ekb.eg/article_65515.html}, eprint = {https://taec.journals.ekb.eg/article_65515_b56705204f79396e644380f0f8b1d740.pdf} } @article { author = {Ellmouni, Faten}, title = {Geometric morphometrics of leaves of Cynanchum acutum L. (Apocynaceae) from Egypt}, journal = {Taeckholmia}, volume = {39}, number = {1}, pages = {86-102}, year = {2019}, publisher = {Cairo University Herbarium (CAI)}, issn = {1110-7413}, eissn = {2357-044X}, doi = {10.21608/taec.2019.19141.1009}, abstract = {This study aimed to revise Cynanchum acutum in Egypt and their relative subspecies that belonging to Section Cynanchum (Old World) and comparing it with an American group of genera Cynanchum laeve subgen. (Sect.) Mellichampia (New World) by using classical taxonomy and Modern geometric morphometric methods (GMMs). The description of 85 specimens depending on 36 quantitative traits was measured on each of the leaves using ImageJ Tool software. Data of leaf descriptors was further processed by using R program. Heatmap appeared to be more fluctuating between two color levels indicated to the similarity and dissimilarity between taxa and population, likewise, a PCA biplot showed a total variation 37.7% at first axis and 8.9% at the second. Significant variation in taxa and populations estimated using ANOVA and MANOVA. Canonical variate analysis showed a significant difference between taxa (P=0.001***), with a total variation of 67% between three taxa. The total variation Checked between two taxa by using discriminant analysis, C. acutum subsp. acutum and C. acutum subsp. sibiricum calculated 77% variation, while as the total variation between then between C. acutum subsp. acutum and C. laeve show 86% of the variation and significant value (P=0.002**). The study elucidated the situation of Cynanchum acutum in Egypt and confirmed the presence of two subspecies viz.: subsp. acutum and subsp. sibiricum}, keywords = {Cynanchum acutum,Modern geometric morphometric methods,leaf architecture,Egypt}, url = {https://taec.journals.ekb.eg/article_65516.html}, eprint = {https://taec.journals.ekb.eg/article_65516_b85f668699cd3e921f2d9bb3fd303761.pdf} } @article { author = {Eldahshan, Omayma and Aly, shaza and Elissawy, Ahmed and Elshanawany, Mohammed and Singab, Abdel Nasser}, title = {Morphological and Genetic Characteristics of Sophora secundiflora and Sophora tomentosa (Fabaceae) cultivated in Egypt}, journal = {Taeckholmia}, volume = {39}, number = {1}, pages = {103-129}, year = {2019}, publisher = {Cairo University Herbarium (CAI)}, issn = {1110-7413}, eissn = {2357-044X}, doi = {10.21608/taec.2020.20572.1010}, abstract = {Genus Sophora L.  belonging to family Fabaceae comprises about 80 species. It is a heterogeneous genus, ranging from tall trees to small, herbaceous plants. Morphological and genetic characteristics of two Sophora species, viz. S. secundiflora (Ortega) DC. and S. tomentosa L.   were subjected to detailed study to delimit these species, where such data are lacking. During this study comparative morphology and anatomy of stem, leaves and petioles of both species was carried out. Moreover, DNA fingerprinting of both plants was carried out using 11 decamer random primers. The obtained results revealed that, many differences in morphological characters viz. the color of the flower, shape of the petiole and leaf dimensions. While the anatomical study reflecting other differential characters among them:   the non-glandular hairs and the dimensions of some microelements. The features of the powdered stems and leaves are also described. Moreover, the DNA fingerprinting; created a total of 232 bands, out of the 99 were polymorphic representing a level of polymorphism of 42.67%. The primer (OPO-02) showed the highest degree of similarities (85.71%), while the lowest (35.29%) was recorded by the primer (OPO-10). This study concludes that the retrieved morphological, anatomical and genetic features provide a powerful identification and characterization tool for both of the studied Sophora species; even when used as powdered material. This study is a pioneer regarding the detailed botanical structure of both species; in addition to, the DNA fingerprinting to distinguish these species; in different prescriptions.  }, keywords = {Botanical profiling,DNA fingerprint,macromorphology,micromorphology,Sophora}, url = {https://taec.journals.ekb.eg/article_68431.html}, eprint = {https://taec.journals.ekb.eg/article_68431_a6080b5d9aed7a2ef024d7ba7d18f667.pdf} }